Got MiLk? (aka First Blog Submission)

Hey,

So in my earlier blog, I was still unclear of what to do for my SRP, but after carefully thinking about it, I have decided research Lactose intolerance

The experiment will involve investigating the biochemical basis of lactose intolerance by adding lactase to lactose and testing for a reaction product, glucose. From my research, I discovered you can do this by testing the glucose concentration levels in milk and lactose free milk and comparing it to the glucose concentration levels in milk and lactose free milk after lactase is added to the milk. The different types of milk is a factor in the experiment.

The materials (milk-lactose and normal), glucose strips, glucose tablets and lactase drops) can easily be obtained from the supermarket or the chemist and because the experiment doesn't involve chemicals or anything potentially dangerous, it can be conducted within household equipment.

So before I conclude whether it is reliable and conducted fairly, I better present the method I will be using. I stated it briefly in the second paragraph, but I left out some stuff.

Firstly, I'll have to test the glucose strips, with positive and negative controls.
By dipping separate glucose strip into the positive (positive solution of 2% water (made by mixing water with the glucose tablet)) and the negative control, (100mls of plain tap water) Hopefully, if nothing goes wrong, I will get a reaction out of the positive control. Following this, I'll put one teaspoon of milk and lactose-free milk in separate cups, and dip the glucose strips to determine the concentration of the milk and compare it, After recording these results, I will add one drop of lactase into the normal milk and lactose free milk, and dip the glucose strips there too, so I can determine the glucose concentration. Then, with the results, I will compare the glucose concentration between regular milk and lactose-free milk before and after the addition to lactase and hope to explain why there is a change in glucose concentration after the addition of lactase in regular and lactose free milk.

The independent variables are the lactose free milk and the normal milk
The dependent variables are the glucose strips
The controlled variables are the amount of milk in each cup, the number of lactase drops and the size of the cups
I not totally sure of this, but I think the Lactase drops would be the catalyst.

So is it.....

Conducted Fairly?
I believe it will be a fair test because I will be repeating it twice, there is a use of a control in my experiment- the glucose strip test- there are a control of variables other than the dependent and independent (listed above).

Reliable?
I will ensure it's reliability by trialing it more than once

Able to be completed within the time frame?
I am certain that it won't take me the time period given to finish the experiment.

Able to give a valid conclusion
determined the level of glucose in normal milk and the level of glucose after the breakdown of lactose? (UNCERTAIN)

oops, I didn't consider this. Might have to change now......sigh. I'll ask ms Zhang about this tomorrow.